Lecture

Mod-01 Lec-17 Advance Analytical Course

This module covers thin layer chromatography (TLC) and high-pressure thin layer chromatography (HPTLC).

Key learning points include:

  • Principles of TLC and HPTLC
  • Comparison of TLC and HPTLC techniques
  • Applications in food and environmental analysis

Students will learn to perform separations and analyze results, emphasizing the practical applications of these techniques in various fields.


Course Lectures
  • This module introduces the foundational concepts of analytical chemistry. Students will learn about the importance of precision and accuracy in analytical measurements.

    Key topics covered include:

    • Basic principles of chromatography and spectroscopy
    • Understanding the role of analytical chemistry in various fields
    • Introduction to laboratory safety and best practices

    By the end of this module, students will have a solid understanding of the key principles that will be explored further in the course.

  • This module focuses on gas chromatography (GC), a vital technique utilized in analytical chemistry. Students will explore various types of detectors used in GC, including:

    • Electron Capture Detector (ECD)
    • Flame Ionization Detector (FID)
    • Thermal Conductivity Detector (TCD)
    • Nitrogen-Phosphorus Detector (NPD)
    • Mass Spectrometry (MS)

    Students will learn the operational principles of each detector, their applications, and advantages in the analysis of volatile compounds.

  • This module dives into high-performance liquid chromatography (HPLC) and reverse-phase liquid chromatography (RPLC). Students will learn about:

    • Principles of HPLC operation and its components
    • Types of stationary and mobile phases
    • Applications in pharmaceutical and environmental analysis

    By the end of this module, students will understand how HPLC can be leveraged for complex mixture analysis and method development.

  • This module covers liquid chromatography with mass detection (LC-MS), a powerful technique used for the identification and quantification of compounds. Students will explore:

    • Principles of LC-MS operation
    • Applications in proteomics and metabolomics
    • Advantages of combining chromatography and mass spectrometry

    Students will gain insights into method development and troubleshooting LC-MS systems for various analytical applications.

  • This module introduces thin layer chromatography (TLC) and high-pressure thin layer chromatography (HPTLC). Key topics include:

    • Basic principles and techniques of TLC
    • Comparison of TLC and HPTLC methodologies
    • Practical applications in food, pharmaceutical, and environmental analysis

    Students will engage in hands-on experiments to reinforce their understanding of these chromatographic techniques.

  • This final module focuses on various spectroscopic techniques including atomic absorption spectroscopy (AAS), inductively coupled plasma emission (ICP/AES), Fourier transform infrared spectrometry (FTIR), and ultraviolet/visible spectrometry (UV/Vis). Key areas of study include:

    • Fundamental principles of each spectroscopy technique
    • Applications in material science, environmental monitoring, and clinical analysis
    • Sample preparation and data interpretation methods

    Students will learn how to select appropriate techniques based on the analytical requirements of different samples.

  • This module delves into the foundational principles of analytical chemistry, focusing on the importance of trace detection techniques.

    Students will explore various separation methods including:

    • Gas Chromatography (GC)
    • High-Performance Liquid Chromatography (HPLC)
    • Thin Layer Chromatography (TLC)

    Real-world applications of these methods will be discussed, providing a practical context for their theoretical underpinnings.

  • In this module, we will focus on various types of detectors used in gas chromatography (GC). Understanding these detectors is crucial for accurate analysis.

    Key topics include:

    • Electron Capture Detector (ECD)
    • Flame Ionization Detector (FID)
    • Thermal Conductivity Detector (TCD)
    • Nitrogen-Phosphorus Detector (NPD)
    • Mass Spectrometer (MS)

    The advantages and limitations of each detector will also be analyzed, helping students choose the right detector for specific applications.

  • This module provides an in-depth look at High-Performance Liquid Chromatography (HPLC) and its applications in analytical chemistry.

    Topics covered include:

    • Principles of HPLC
    • Components of HPLC systems
    • Applications in pharmaceuticals, food safety, and environmental testing

    Students will engage in hands-on activities to reinforce their understanding of HPLC techniques and methods.

  • This module focuses on Liquid Chromatography-Mass Spectrometry (LC-MS), a powerful analytical technique that combines the separation capabilities of chromatography with mass spectrometry.

    Key aspects include:

    • Principles of LC-MS
    • Applications in bioanalysis and proteomics
    • Advantages over traditional methods

    Students will learn how to interpret LC-MS data and understand its significance in research and industry.

  • This module introduces students to Thin Layer Chromatography (TLC) and High-Pressure Thin Layer Chromatography (HPTLC), two essential techniques in analytical chemistry.

    Students will cover:

    • The principles of TLC and HPTLC
    • Applications in qualitative analysis
    • Advantages of HPTLC over traditional TLC

    Hands-on lab sessions will allow students to practice these methods and analyze real samples effectively.

  • This module covers various spectroscopy techniques used in analytical chemistry, including Atomic Absorption Spectroscopy (AAS) and Fourier Transform Infrared Spectrometry (FTIR).

    Students will explore:

    • The principles of AAS and its applications in metal analysis
    • FTIR and its role in identifying molecular structures
    • Ultraviolet/Visible Spectrometry (UV/Vis) and its applications in chemical analysis

    Students will also engage in practical experiments to reinforce their understanding of these techniques.

  • This module introduces the foundational concepts of analytical chemistry, focusing on the importance of trace detection techniques.

    Key topics include:

    • Overview of analytical chemistry
    • Understanding trace analysis
    • Introduction to chromatography and spectroscopy

    Students will learn about the significance of accurate measurements and how these techniques apply to real-world scenarios in various industries.

  • This module delves into gas chromatography (GC) and its various detectors.

    Topics covered include:

    • Principles of gas chromatography
    • Types of detectors: ECD, FID, TCD, NPD, and MS
    • Applications of GC in different fields

    Students will gain practical skills in utilizing GC for complex sample analysis, understanding the benefits and limitations of each detector type.

  • This module focuses on high-performance liquid chromatography (HPLC) and reverse-phase liquid chromatography (RPLC).

    Key elements include:

    • Principles of HPLC
    • Understanding RPLC techniques
    • Applications of liquid chromatography in pharmaceuticals and environmental analysis

    Through practical examples, students will learn about method development and optimization for various applications.

  • This module introduces liquid chromatography with mass detection (LC-MS) and its significance in analytical chemistry.

    Topics discussed include:

    • Principles of LC-MS operation
    • Applications in biochemistry and pharmaceutical analysis
    • Advantages of combining chromatography with mass detection

    Students will explore the integration of LC-MS in complex sample analysis, enhancing their understanding of modern analytical techniques.

  • This module covers thin layer chromatography (TLC) and high-pressure thin layer chromatography (HPTLC).

    Key learning points include:

    • Principles of TLC and HPTLC
    • Comparison of TLC and HPTLC techniques
    • Applications in food and environmental analysis

    Students will learn to perform separations and analyze results, emphasizing the practical applications of these techniques in various fields.

  • This module focuses on atomic absorption spectroscopy (AAS) and inductively coupled plasma emission spectroscopy (ICP/AES).

    Topics include:

    • Principles of AAS and ICP/AES
    • Applications in elemental analysis
    • Advantages and limitations of each technique

    Students will learn to utilize these spectroscopy methods for quantifying trace elements in various samples, enhancing their practical laboratory skills.

  • This module introduces the foundational principles of analytical chemistry, focusing on the significance of analytical techniques in various fields. Students will explore:

    • The role of chromatography in separating and analyzing mixtures.
    • The principles behind spectroscopy and its applications in identifying substances.
    • Understanding the various types of detectors used in gas chromatography.

    By the end of this module, learners will have a solid understanding of how these analytical methods are applied in real-world scenarios, paving the way for more advanced topics in subsequent modules.

  • In this module, students will delve deeper into gas chromatography (GC) and its various detectors, including:

    • Electron Capture Detector (ECD)
    • Flame Ionization Detector (FID)
    • Thermal Conductivity Detector (TCD)
    • Nitrogen-Phosphorus Detector (NPD)
    • Mass Spectrometry (MS)

    Students will learn about the operational principles, advantages, and limitations of each type of detector, as well as practical applications in environmental and pharmaceutical analysis.

  • This module covers high-performance liquid chromatography (HPLC) and its significance in analytical chemistry. Key topics include:

    • Fundamentals of HPLC operation and instrumentation.
    • Types of chromatography including reverse-phase liquid chromatography (RPLC).
    • Applications of HPLC in various industries such as pharmaceuticals and food safety.

    Students will engage in hands-on activities to gain practical experience in setting up and conducting HPLC analyses.

  • This module introduces liquid chromatography coupled with mass detection (LC-MS), a powerful technique in the field of analytical chemistry. Topics include:

    • Principles of operation of LC-MS.
    • Advantages of combining liquid chromatography with mass spectrometry.
    • Applications in clinical diagnostics and environmental monitoring.

    Students will learn how LC-MS is utilized for qualitative and quantitative analysis, enhancing their understanding of modern analytical techniques.

  • This module focuses on thin layer chromatography (TLC) and its advanced counterpart, high pressure thin layer chromatography (HPTLC). Key aspects covered include:

    • Basic principles of TLC and its applications.
    • Operational differences between TLC and HPTLC.
    • Analyzing the efficiency and speed of HPTLC in comparison to traditional methods.

    Students will engage in practical sessions to apply these techniques in real-world scenarios, enhancing their analytical skills.

  • This module provides an overview of various spectroscopic techniques, focusing on:

    • Atomic Absorption Spectroscopy (AAS)
    • Inductively Coupled Plasma Emission Spectroscopy (ICP/AES)
    • Fourier Transform Infrared Spectrometry (FTIR)
    • Ultraviolet/Visible Spectrometry (UV/Vis)

    Students will learn about the principles behind each technique, their applications in material characterization, and how to interpret spectroscopic data.

  • In this module, students will be introduced to the fundamental concepts of gas chromatography (GC). This includes understanding the principles behind GC and the various types of detectors used in the process. The module will cover:

    • Electron Capture Detector (ECD)
    • Flame Ionization Detector (FID)
    • Thermal Conductivity Detector (TCD)
    • Nitrogen-Phosphorus Detector (NPD)
    • Mass Spectrometry (MS) integration with GC

    By the end of this module, students will gain practical knowledge on the applications of GC in real-world scenarios, paving the way for advanced analytical techniques.

  • This module focuses on high-performance liquid chromatography (HPLC) and its various applications in analytical chemistry. Students will learn about:

    • The principles of HPLC and its advantages over traditional liquid chromatography.
    • Reverse-phase liquid chromatography (RPLC) and its significance.
    • Techniques for method development and optimization.
    • Real-life applications of HPLC in pharmaceutical and environmental analysis.

    Students will engage in practical exercises to solidify their understanding of HPLC methods and their role in modern analytical laboratories.

  • This module delves into thin-layer chromatography (TLC) and high-pressure thin-layer chromatography (HPTLC). Key topics include:

    • Theoretical foundations of TLC and HPTLC techniques.
    • Comparative analysis of TLC and HPTLC methods.
    • Applications of TLC in various fields, including food and pharmaceutical industries.
    • Hands-on training with TLC plates and visualization methods.

    Students will develop practical skills necessary for conducting TLC and HPTLC experiments, along with interpreting results effectively.

  • In this module, students will explore atomic absorption spectroscopy (AAS) and inductively coupled plasma emission spectroscopy (ICP/AES). The topics covered include:

    • Basic principles of AAS and its applications in elemental analysis.
    • The working mechanism of ICP and its advantages over AAS.
    • Sample preparation techniques for both AAS and ICP.
    • Case studies demonstrating the use of these techniques in environmental monitoring.

    This module will enable students to understand the significance of these techniques in detecting trace elements in various matrices.

  • This module provides an in-depth look at Fourier transform infrared spectrometry (FTIR) and ultraviolet/visible spectrometry (UV/Vis). Key learning areas include:

    • Fundamental principles of FTIR and UV/Vis spectroscopy.
    • Instrumentation and operational techniques for both methods.
    • Applications in identifying organic compounds and analyzing chemical structures.
    • Hands-on practice with FTIR and UV/Vis spectrometers.

    Students will gain valuable skills in spectral interpretation and the application of these techniques in various industries.

  • This final module integrates all the analytical techniques covered throughout the course. Students will engage in case studies that demonstrate:

    • Real-world applications of chromatography and spectroscopy techniques.
    • Comparative analysis and method selection based on specific analytical needs.
    • Hands-on lab sessions to apply the knowledge gained from prior modules.
    • Collaboration on group projects to solve complex analytical challenges.

    By the end of this module, students will be well-equipped to approach analytical problems using a variety of techniques and make informed decisions in laboratory settings.

  • This module introduces the fundamental principles of chromatographic techniques, with a focus on gas chromatography (GC). Students will learn about various detector types, including:

    • Electron Capture Detector (ECD)
    • Flame Ionization Detector (FID)
    • Thermal Conductivity Detector (TCD)
    • Nitrogen-Phosphorus Detector (NPD)
    • Mass Spectrometry (MS)

    Through practical examples, participants will understand the applications and advantages of each detector type, setting a solid foundation for advanced analytical techniques.

  • This module delves into high-performance liquid chromatography (HPLC) and reverse-phase liquid chromatography (RPLC). Key topics include:

    • The principles and mechanisms of HPLC
    • Comparison between normal and reverse-phase chromatography
    • Applications of HPLC in various fields

    Students will gain hands-on experience in setting up HPLC experiments and interpreting chromatograms, enhancing their practical skills in analytical chemistry.

  • This module focuses on liquid chromatography coupled with mass spectrometry (LC-MS). Students will learn:

    • The integration of liquid chromatography with mass spectrometry
    • Advantages of LC-MS in complex sample analysis
    • Applications in pharmaceutical and environmental analysis

    Through case studies, participants will understand how LC-MS improves sensitivity and specificity in analytical measurements.

  • This module covers thin layer chromatography (TLC) and high-pressure thin layer chromatography (HPTLC). Key elements include:

    • Basics of TLC and its applications
    • Advantages of HPTLC over traditional TLC
    • Quantitative and qualitative analysis using TLC techniques

    Students will engage in practical sessions to develop their skills in characterizing compounds and evaluating separation efficiency.

  • This module introduces atomic absorption spectroscopy (AAS) and inductively coupled plasma emission spectroscopy (ICP/AES). Key topics include:

    • The principles of atomic absorption and its applications
    • How ICP/AES enhances elemental analysis
    • Comparative advantages of these techniques in various fields

    Practical demonstrations will allow students to experience the setup and execution of AAS and ICP/AES experiments, focusing on real-world applications.

  • This module provides an overview of Fourier transform infrared spectrometry (FTIR) and ultraviolet/visible spectrometry (UV/Vis). Students will explore:

    • The principles underlying FTIR and its application in compound identification
    • How UV/Vis spectrometry is used for quantitative analysis
    • Comparative analysis of both spectrometric techniques

    Hands-on exercises will enable students to interpret spectra and understand the practical applications of these spectrometric methods in various research fields.

  • This module introduces students to the fundamental principles of analytical chemistry, focusing on various detection techniques.

    Key topics covered include:

    • Chromatography fundamentals
    • Gas chromatography (GC) with various detectors
    • High-performance liquid chromatography (HPLC)
    • Thin-layer chromatography (TLC)

    Students will gain insights into the applications of these techniques in real-world scenarios, enhancing their understanding of analytical methods.

  • This module delves into the advanced techniques of gas chromatography (GC), exploring the various types of detectors available.

    Topics include:

    • Electron capture detectors (ECD)
    • Flame ionization detectors (FID)
    • Thermal conductivity detectors (TCD)
    • Nitrogen-Phosphorus detectors (NPD)
    • Mass spectrometry (MS) integration

    Students will engage in practical applications, learning how to select the appropriate detector for specific chemical analyses.

  • This module focuses on high-performance liquid chromatography (HPLC) and its various configurations, including reverse-phase liquid chromatography (RPLC).

    Key learning points include:

    • Principles of HPLC operation
    • Advantages and limitations of RPLC
    • Applications of HPLC in different fields
    • Hands-on experience with LC-MS techniques

    Students will engage in practical laboratory sessions to reinforce the concepts learned and apply them to real-world scenarios.

  • This module provides an overview of spectroscopy techniques, including atomic absorption spectroscopy (AAS) and inductively coupled plasma (ICP/AES).

    Students will learn about:

    • Fundamentals of AAS and its applications
    • Principles of ICP/AES
    • Fourier transform infrared spectrometry (FTIR)
    • Ultraviolet/visible (UV/Vis) spectrometry

    Practical sessions will enable students to conduct analyses using these techniques, preparing them for careers in analytical chemistry.